Nodal Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13664
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Nodal Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: PTEGSLAIEIFHQPKPDTEQASDSCLERFQMDLFTVTLSQVTFSLGSMVLEVTRPLSKWLKHPGALEKQMSRV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nodal Antibody
![Western Blot: Nodal Antibody [NBP2-13664]](https://resources.bio-techne.com/images/products/Nodal-Antibody-Western-Blot-NBP2-13664-img0003.jpg "Western Blot: Nodal Antibody [NBP2-13664]")
Western Blot: Nodal Antibody [NBP2-13664]
Western Blot: Nodal Antibody [NBP2-13664] - Analysis in control (vector only transfected HEK293T lysate) and NODAL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: Nodal Antibody [NBP2-13664]](https://resources.bio-techne.com/images/products/Nodal-Antibody-Immunohistochemistry-Paraffin-NBP2-13664-img0001.jpg "Immunohistochemistry-Paraffin: Nodal Antibody [NBP2-13664]")
Immunohistochemistry-Paraffin: Nodal Antibody [NBP2-13664]
Immunohistochemistry-Paraffin: Nodal Antibody [NBP2-13664] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.Applications for Nodal Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nodal
Alternate Names
BMP-16
Gene Symbol
NODAL
Additional Nodal Products
Product Documents for Nodal Antibody
Product Specific Notices for Nodal Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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