Nogo Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47354

Novus Biologicals, part of Bio-Techne
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NBP2-47354
NBP2-47354-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nogo Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ETEAPYISIACDLIKETKLSAEPAPDFSDYSEMAKVEQPVPDHSELVEDSSPDSEPVDLFSDDSIPDVPQKQDETVMLVKESLTETSFESMIEYENKEKLSALPPEGGKPYLESFKLSLDNTKDTLLPDEVSTLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nogo Antibody

Immunocytochemistry/ Immunofluorescence: Nogo Antibody [NBP2-47354]

Immunocytochemistry/ Immunofluorescence: Nogo Antibody [NBP2-47354]

Immunocytochemistry/Immunofluorescence: Nogo Antibody [NBP2-47354] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nogo Antibody [NBP2-47354]

Immunohistochemistry-Paraffin: Nogo Antibody [NBP2-47354]

Immunohistochemistry-Paraffin: Nogo Antibody [NBP2-47354] - Staining of human testis shows strong cytoplasmic positivity in seminiferous ducts cells.

Applications for Nogo Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nogo

Neurite Outgrowth Inhibitor (NOGO) is a myelin associated neurite growth inhibitor found in oligodendrocyte and central nervous system (CNS) myelin membranes of the adult CNS. NOGO plays a role in blocking axonal regeneration in the CNS of higher vertebrates. At least 6 isoforms have been observed arising from alternative splicing. NOGO-A (Isoform 1) is specifically expressed in brain and testes and weakly expressed in heart and skeletal muscle.

Alternate Names

ASY, foocen, Human NogoA, KIAA0886, My043 protein, Nbla00271, Nbla10545, neurite growth inhibitor 220, Neurite outgrowth inhibitor, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, NI220/250, Nogo protein, NOGO-A, Nogo-B, NOGOC, Nogo-C, NOGORTN4-B1, NSP, NSP-CL, reticulon 4, reticulon 5, reticulon-4, Reticulon-5, RTN4-A, RTN4-B2, RTN4-C, RTN-x

Gene Symbol

RTN4

Additional Nogo Products

Product Documents for Nogo Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nogo Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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