NOLA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31742

Novus Biologicals, part of Bio-Techne
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NBP2-31742-25ul
NBP2-31742
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NOLA1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GRGGFNKGQDQGPPERVVLLGEFLHPCEDDIVCKCTT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOLA1 Antibody

Immunocytochemistry/ Immunofluorescence: NOLA1 Antibody [NBP2-31742]

Immunocytochemistry/ Immunofluorescence: NOLA1 Antibody [NBP2-31742]

Immunocytochemistry/Immunofluorescence: NOLA1 Antibody [NBP2-31742] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742]

Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742]

Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742]

Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742]

Immunohistochemistry-Paraffin: NOLA1 Antibody [NBP2-31742] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
View 3 more images

Applications for NOLA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

Reacivity reported in (PMID: 29695869)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOLA1

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex. which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5. instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues. which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC. the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Alternate Names

GAR1 ribonucleoprotein homolog (yeast), H/ACA ribonucleoprotein complex subunit 1, NOLA1member 1 (H/ACA small nucleolar RNPs), Nucleolar protein family A member 1, snoRNP protein GAR1

Gene Symbol

GAR1

UniProt

Q9NY12 (Human)

Additional NOLA1 Products

Product Documents for NOLA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NOLA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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