non-muscle heavy chain 10 Myosin Antibody (GT246)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13591

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-13591
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT246

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all non-muscle heavy chain 10 Myosin Antibodies »

Product Summary for non-muscle heavy chain 10 Myosin Antibody (GT246)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human non-muscle heavy chain 10 Myosin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

229 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for non-muscle heavy chain 10 Myosin Antibody (GT246)

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Western Blot: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunocytochemistry/ Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunocytochemistry/Immunofluorescence: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: non-muscle heavy chain 10 Myosin protein stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591]

Immunohistochemistry-Frozen: non-muscle heavy chain 10 Myosin Antibody (GT246) [NBP3-13591] - non-muscle heavy chain 10 Myosin antibody [GT246] detects non-muscle heavy chain 10 Myosin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: non-muscle heavy chain 10 Myosin stained by non-muscle heavy chain 10 Myosin antibody [GT246] (NBP3-13591) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
View 4 more images

Applications for non-muscle heavy chain 10 Myosin Antibody (GT246)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: non-muscle heavy chain 10 Myosin

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Long Name

Myosin-10

Alternate Names

MYH10, NMMHC II-b, NMMHC-B, NMMHC-IIB

Gene Symbol

MYH10

Additional non-muscle heavy chain 10 Myosin Products

Product Documents for non-muscle heavy chain 10 Myosin Antibody (GT246)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for non-muscle heavy chain 10 Myosin Antibody (GT246)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.