Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all non-muscle heavy chain 10 Myosin Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1955 and 1976 of human myosin, heavy chain 10, non-muscle using the numbering given in entry NP_005955.1 (GeneID 4628).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP1-18932]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP1-18932]

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP1-18932] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18931.

Applications for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: non-muscle heavy chain 10 Myosin

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Long Name

Myosin-10

Alternate Names

MYH10, NMMHC II-b, NMMHC-B, NMMHC-IIB

Entrez Gene IDs

4628 (Human); 77579 (Mouse)

Gene Symbol

MYH10

UniProt

P35580 (Human)

Additional non-muscle heavy chain 10 Myosin Products

Product Documents for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for non-muscle heavy chain 10 Myosin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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