NOP58 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81680

Novus Biologicals, part of Bio-Techne
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NBP1-81680
NBP1-81680-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NOP58 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAIAPNVTVMVGELVGARLIAHAGSLL

Marker

Nucleolar Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOP58 Antibody

Western Blot: NOP58 Antibody [NBP1-81680]

Western Blot: NOP58 Antibody [NBP1-81680]

Western Blot: NOP58 Antibody [NBP1-81680] - Analysis using Anti-NOP58 antibody NBP1-81680 (A) shows similar pattern to independent antibody NBP1-81681 (B).
Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81680]

Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81680]

Immunocytochemistry/Immunofluorescence: NOP58 Antibody [NBP1-81680] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680]

Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680]

Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81680] - Staining of human stomach shows strong nucleolar and moderate nuclear positivity in glandular cells.
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Applications for NOP58 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOP58

Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).

Alternate Names

HSPC120, NOL5, NOP5/NOP58, NOP58 ribonucleoprotein homolog (yeast), NOP5nucleolar protein 58, Nucleolar protein 5, nucleolar protein NOP5/NOP58

Gene Symbol

NOP58

Additional NOP58 Products

Product Documents for NOP58 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NOP58 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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