NOP58 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81681
![Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81681]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Immunohistochemistry-Paraffin-NBP1-81681-img0007.jpg "Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-81681]")
![Western Blot: NOP58 Antibody [NBP1-81681]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Western-Blot-NBP1-81681-img0011.jpg "Western Blot: NOP58 Antibody [NBP1-81681]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTAEALAAFTALMEGKINKQLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLSCIHSPVVNELMRGIRSQMDGL
Marker
Nucleolar Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOP58 Antibody
![Western Blot: NOP58 Antibody [NBP1-81681]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Western-Blot-NBP1-81681-img0010.jpg "Western Blot: NOP58 Antibody [NBP1-81681]")
Western Blot: NOP58 Antibody [NBP1-81681]
Western Blot: NOP58 Antibody [NBP1-81681] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81681]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81681-img0009.jpg "Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81681]")
Immunocytochemistry/ Immunofluorescence: NOP58 Antibody [NBP1-81681]
Immunocytochemistry/Immunofluorescence: NOP58 Antibody [NBP1-81681] - Staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.Applications for NOP58 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NOP58
Alternate Names
HSPC120, NOL5, NOP5/NOP58, NOP58 ribonucleoprotein homolog (yeast), NOP5nucleolar protein 58, Nucleolar protein 5, nucleolar protein NOP5/NOP58
Gene Symbol
NOP58
Additional NOP58 Products
Product Documents for NOP58 Antibody
Product Specific Notices for NOP58 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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