Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Notum Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM. The immunogen is located within amino acids 90 - 140 of NOTUM. Amino Acid Squence: CNDGSPAGYYLKESRGSRR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOTUM Antibody - BSA Free

Western Blot: NOTUM AntibodyBSA Free [NBP1-77060]

Western Blot: NOTUM AntibodyBSA Free [NBP1-77060]

Western Blot: NOTUM Antibody [NBP1-77060] - Human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: NOTUM Antibody - BSA Free [NBP1-77060]

Immunocytochemistry/ Immunofluorescence: NOTUM Antibody - BSA Free [NBP1-77060]

Immunocytochemistry/Immunofluorescence: NOTUM Antibody [NBP1-77060] - Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: NOTUM Antibody - BSA Free [NBP1-77060]

Immunohistochemistry-Paraffin: NOTUM Antibody - BSA Free [NBP1-77060]

Immunohistochemistry-Paraffin: NOTUM Antibody [NBP1-77060] - Human brain tissue with NOTUM antibody at 2.5 ug/ml.
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Applications for NOTUM Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Notum

In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

Alternate Names

EC 3.-, notum pectinacetylesterase homolog (Drosophila), protein notum homolog

Gene Symbol

NOTUM

UniProt

NP_848588 (Human)

Additional Notum Products

Product Documents for NOTUM Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NOTUM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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