NOV/CCN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31416

Novus Biologicals, part of Bio-Techne
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NBP1-31416
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NOV/CCN3 Antibodies »

Product Summary for NOV/CCN3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOV/CCN3. The exact sequence is proprietary.

Reactivity Notes

Rat (83%), Bovine (82%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NOV/CCN3 Antibody

Western Blot: NOV/CCN3 Antibody [NBP1-31416]

Western Blot: NOV/CCN3 Antibody [NBP1-31416]

Western Blot: NOV/CCN3 Antibody [NBP1-31416] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-31416]

Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-31416]

Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-31416] - Analysis of HeLa, using NBP1-31416 at 1:200 dilution.
Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-31416]

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-31416]

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-31416] - Sastric cancer N87 xenograft, using NOV antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for NOV/CCN3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NOV/CCN3

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Long Name

Nephroblastoma Overexpressed Gene

Alternate Names

CCN3, IGFBP-9

Entrez Gene IDs

4856 (Human); 18133 (Mouse)

Gene Symbol

CCN3

UniProt

P48745

Additional NOV/CCN3 Products

Product Documents for NOV/CCN3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NOV/CCN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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