NOV/CCN3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88154

Novus Biologicals, part of Bio-Techne
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NBP1-88154
NBP1-88154-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NOV/CCN3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSNQTGICTAVEGDNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGC

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOV/CCN3 Antibody

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-88154]

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-88154]

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-88154] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding NOV RNA-seq data are presented for the same tissues.
Western Blot: NOV/CCN3 Antibody [NBP1-88154]

Western Blot: NOV/CCN3 Antibody [NBP1-88154]

Western Blot: NOV/CCN3 Antibody [NBP1-88154] - Analysis in control (vector only transfected HEK293T lysate) and NOV over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88154]

Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88154]

Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-88154] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
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Applications for NOV/CCN3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOV/CCN3

CCN3 is encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Long Name

Nephroblastoma Overexpressed Gene

Alternate Names

CCN3, IGFBP-9

Gene Symbol

CCN3

Additional NOV/CCN3 Products

Product Documents for NOV/CCN3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NOV/CCN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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