Nova1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00221

Novus Biologicals, part of Bio-Techne
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NBP1-00221
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Nova1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-REMPQNVAKTEPVS corresponding to internal region according to NP_002506.2 , NP_006480.2 , NP_006482.1.

Specificity

This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nova1 Antibody

Western Blot: Nova1 Antibody [NBP1-00221]

Western Blot: Nova1 Antibody [NBP1-00221]

Western Blot: Nova1 Antibody [NBP1-00221] - HEK293 overexpressing NOVA1 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221]

Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221]

Immunohistochemistry-Paraffin: Nova1 Antibody [NBP1-00221] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Nova1 Antibody [NBP1-00221]

Western Blot: Nova1 Antibody [NBP1-00221]

Western Blot: Nova1 Antibody [NBP1-00221] - Analysis of Nova1 in Human Breast Cancer lysate (35 ug protein in RIPA buffer) using NBP1-00221 at 0.01 ug/mL. Detected by chemiluminescence.
View 2 more images

Applications for Nova1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
WB: In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human cerebral cortex shows staining of selected nuclei.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nova1

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq]

Alternate Names

neuro-oncological ventral antigen 1Nova-1, Onconeural ventral antigen 1, Paraneoplastic Ri antigen, RNA-binding protein Nova-1, Ventral neuron-specific protein 1

Entrez Gene IDs

4857 (Human)

Gene Symbol

NOVA1

UniProt

NP_002506.2 (Human)

Additional Nova1 Products

Product Documents for Nova1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nova1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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