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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Western Blot

Label

Janelia Fluor 585

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NOX4/1245

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant human Nox4 protein (Uniprot: Q9NPH5)

Localization

Cytoplasm and Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for Nox4 Antibody (NOX4/1245) [Janelia Fluor® 585]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nox4

NOX4 (RENOX) is a renal gp91-phox homolog which functions in the kidney as an oxygen sensor and regulates the synthesis of erythropoietin. This protein is highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex.

NOX-4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of NOX4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases NOX4 expression, which indicates that NOX4 has a role in vascular oxidative stress response. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggest that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.

Long Name

NADPH Oxidase 4

Alternate Names

KOX-1, RENOX

Gene Symbol

NOX4

Additional Nox4 Products

Product Documents for Nox4 Antibody (NOX4/1245) [Janelia Fluor® 585]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nox4 Antibody (NOX4/1245) [Janelia Fluor® 585]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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