NOX5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68862

Novus Biologicals, part of Bio-Techne
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NBP1-68862
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NOX5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EWHPFTISSAPEQKD corresponding to internal region according to NP_078781.3, NP_001171708.1, NP_001171709.1.

Epitope

C-EWHPFTISSAPEQKD

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32165681).

Specificity

This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NOX5 Antibody

Western Blot: NOX5 Antibody [NBP1-68862]

Western Blot: NOX5 Antibody [NBP1-68862]

Western Blot: NOX5 Antibody [NBP1-68862] - NBP1-68862 (0.5ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane . The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/ Immunofluorescence: NOX5 Antibody [NBP1-68862]

Immunocytochemistry/Immunofluorescence: NOX5 Antibody [NBP1-68862] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing staining within the cytoplasm and plasma membrane . The nuclear stain is DAPI (blue).Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NOX5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human testis lysates (calculated MW of 86.4 kDa band according to NP_078781.3).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NOX5

NOX5 is a novel NADPH oxidase that generates superoxide and functions as a H+ channel in a Ca(2+)-dependentmanner.(supplied by OMIM)

Long Name

NADPH oxidase 5

Alternate Names

EC 1.6.3.-, MGC149776, MGC149777, NADPH oxidase 5, NADPH oxidase, EF-hand calcium binding domain 5, NOX5A, NOX5B

Entrez Gene IDs

79400 (Human)

Gene Symbol

NOX5

UniProt

NP_078781.3 (Human)

Additional NOX5 Products

Product Documents for NOX5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NOX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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