Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S408-79

Concentration

1 mg/ml

View all Npas4 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasm , Nucleus

Specificity

Detects 90kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Npas4 Antibody (S408-79)

Western Blot: Npas4 Antibody (S408-79) [NBP2-59332]

Western Blot: Npas4 Antibody (S408-79) [NBP2-59332]

Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.
Immunocytochemistry/ Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332]

Immunocytochemistry/ Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332]

Immunocytochemistry/Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

Applications for Npas4 Antibody (S408-79)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 31041873).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Npas4

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Alternate Names

BHLHE79, bHLHe79neuronal PAS domain-containing protein 4, Class E basic helix-loop-helix protein 79, Le-PAS, neuronal PAS domain protein 4, Neuronal PAS4, NXFHLH-PAS transcription factor NXF, PASD10PAS domain-containing protein 10

Gene Symbol

NPAS4

Additional Npas4 Products

Product Documents for Npas4 Antibody (S408-79)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Npas4 Antibody (S408-79)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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