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Npas4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68863

Novus Biologicals, part of Bio-Techne
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NBP1-68863

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence CRFNTSKSLRRQS corresponding to internal region according to NP_849195.2.

Epitope

CRFNTSKSLRRQS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Npas4 Antibody

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863] - Staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863] - NBP1-68863 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Npas4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human brain (frontal cortex) lysates and in mouse and rat brain lysates (calculated MW of 87.1 kDa band according to NP_849195.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Npas4

NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM]

Alternate Names

BHLHE79, bHLHe79neuronal PAS domain-containing protein 4, Class E basic helix-loop-helix protein 79, Le-PAS, neuronal PAS domain protein 4, Neuronal PAS4, NXFHLH-PAS transcription factor NXF, PASD10PAS domain-containing protein 10

Entrez Gene IDs

266743 (Human); 225872 (Mouse); 266734 (Rat)

Gene Symbol

NPAS4

Additional Npas4 Products

Product Documents for Npas4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Npas4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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