NPM1 Antibody (NPM1/3287)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79741

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NPM1/3287

Concentration

0.2 mg/ml

View all NPM1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa185-287) of human NPM1 protein (exact sequence is proprietary) (Uniprot: P06748 )

Localization

Nuclear, Cytoplasmic

Specificity

Recognizes a 33kDa glycoprotein, identified as NPM1 (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Marker

Acute Myeloid Leukemia Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79876)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for NPM1 Antibody (NPM1/3287)

Western Blot: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Western Blot: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Western Blot: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Western Blot Analysis of K562 cell lysate using NPM1 Antibody (NPM1/3287).
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Immunocytochemistry/Immunofluorescence: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Immunofluorescence staining of HeLa cells using NPM1 antibody (NPM1/3287) followed by GAM IgG-CF488 (green). Membrane stained with phalloidin (red).
Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3287) [NBP2-79741]

Immunohistochemistry-Paraffin: NPM1 Antibody (NPM1/3287) [NBP2-79741] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with NPM1 Antibody (NPM1/3287).
View 2 more images

Applications for NPM1 Antibody (NPM1/3287)

Application
Recommended Usage

ELISA

2-4 ug/ml

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NPM1

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

UniProt

P06748

Additional NPM1 Products

Product Documents for NPM1 Antibody (NPM1/3287)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NPM1 Antibody (NPM1/3287)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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