NPM1 Antibody (rNPM1/1901) [Janelia Fluor® 549]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08750JF549
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry-Paraffin, Western Blot
Label
Janelia Fluor 549
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rNPM1/1901
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary) (Uniprot: P06748)
Localization
Nuclear and Cytoplasmic
Specificity
Recognizes a 33kDa glycoprotein, identified as NPM1 (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Marker
Acute Myeloid Leukemia Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for NPM1 Antibody (rNPM1/1901) [Janelia Fluor® 549]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: NPM1
NMP antibodies are useful tools as tumor markers in cancer research.
Long Name
Nucleophosmin/Nucleolar Phosphoprotein B23
Alternate Names
B23, Nucleophosmin, Numatrin
Gene Symbol
NPM1
Additional NPM1 Products
Product Documents for NPM1 Antibody (rNPM1/1901) [Janelia Fluor® 549]
Product Specific Notices for NPM1 Antibody (rNPM1/1901) [Janelia Fluor® 549]
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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