NR4A3/NOR1 Antibody (1E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00008013-M02

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

NR4A3 (NP_008912, 414 a.a. ~ 521 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLLIESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFS

Specificity

NR4A3 - nuclear receptor subfamily 4, group A, member 3 (1E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NR4A3/NOR1 Antibody (1E9)

Western Blot: NR4A3/NOR1 Antibody (1E9) [H00008013-M02]

Western Blot: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - NR4A3 monoclonal antibody (M02), clone 1E9 Analysis of NR4A3 expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: NR4A3/NOR1 Antibody (1E9) [H00008013-M02]

Immunocytochemistry/Immunofluorescence: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - Analysis of monoclonal antibody to NR4A3 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - Detection limit for recombinant GST tagged NR4A3 is approximately 0.3ng/ml as a capture antibody.

Applications for NR4A3/NOR1 Antibody (1E9)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NR4A3/NOR1

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Four transcript variants encoding three distinct isoforms have been identified for this gene.

Alternate Names

CHN, CSMF, MINOR, NOR1, TEC

Entrez Gene IDs

8013 (Human)

Gene Symbol

NR4A3

UniProt

NP_008912 (Human)

Additional NR4A3/NOR1 Products

Product Documents for NR4A3/NOR1 Antibody (1E9)

Product Datasheets

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COA

SDS

Product Specific Notices for NR4A3/NOR1 Antibody (1E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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