NRAMP2/SLC11A2/DMT1 Antibody (4C6)
Novus Biologicals, part of Bio-Techne | Catalog # H00004891-M01
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Porcine, Bovine
Cited:
Human, Bovine
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4C6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SLC11A2 (NP_000608, 1 a.a. ~ 65 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYSCF
Reactivity Notes
Bovine reactivity reported in scientific literature (PMID: 19820055).
Specificity
SLC11A2 - solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NRAMP2/SLC11A2/DMT1 Antibody (4C6)
Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01]
Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Immunohistochemistry of iron handling proteins in healthy kidney. Representatives images of ZIP8 (a), ZIP14 (b), divalent metal transporter 1 (DMT1; c), L-ferritin (d), H-ferritin (e), and ferroportin (f) staining in healthy kidney. Renal structures indicated as glomerulus (g), proximal tubule (PT), distal tubule (DT). Scale bar 20uM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-27107-8) licensed under a CC-BY license.Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01]
Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Analysis of monoclonal antibody to SLC11A2 on formalin-fixed paraffin-embedded human endometrium cancer. Antibody concentration 3 ug/ml.ELISA: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01]
ELISA: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Detection limit for recombinant GST tagged SLC11A2 is 0.03 ng/ml as a capture antibody.Applications for NRAMP2/SLC11A2/DMT1 Antibody (4C6)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
It has been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 29921869).
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NRAMP2/SLC11A2
Long Name
Natural Resistance-associated Macrophage Protein 2
Alternate Names
DCT1, SLC11A2
Entrez Gene IDs
4891 (Human)
Gene Symbol
SLC11A2
OMIM
206 (Human)
UniProt
Additional NRAMP2/SLC11A2 Products
Product Documents for NRAMP2/SLC11A2/DMT1 Antibody (4C6)
Product Specific Notices for NRAMP2/SLC11A2/DMT1 Antibody (4C6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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