Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Guinea Pig (93%), Porcine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all NRAS Antibodies »

Product Specifications

Immunogen

NRAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human NRAS. The immunogen is located within the last 50 amino acids of NRAS. Amino Acid Squence: QYRMKKLNSSDDGTQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (80%)

Specificity

NRAS antibody is predicted to not cross-react with H-RAS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NRAS Antibody - BSA Free

Western Blot: NRAS AntibodyBSA Free [NBP2-81861]

Western Blot: NRAS AntibodyBSA Free [NBP2-81861]

Western Blot: NRAS Antibody [NBP2-81861] - Western blot analysis of NRAS in A431 cell lysate with NRAS antibody at (A) 1 and (B) 2 ug/mL.

Applications for NRAS Antibody - BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NRAS

Ras (p21), a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30% of all human cancer (4).

Alternate Names

ALPS4, GTPase NRas, HRAS1, neuroblastoma RAS viral (v-ras) oncogene homolog, N-ras, N-ras protein part 4, NRAS1, NS6, Transforming protein N-Ras, v-ras neuroblastoma RAS viral oncogene homolog

Gene Symbol

NRAS

Additional NRAS Products

Product Documents for NRAS Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NRAS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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