Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NRBF2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NRBF2 (NP_110386) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NRBF2 Antibody (OTI1A2) - Azide and BSA Free

Western Blot: NRBF2 Antibody (OTI1A2)Azide and BSA Free [NBP2-73054]

Western Blot: NRBF2 Antibody (OTI1A2)Azide and BSA Free [NBP2-73054]

Western Blot: NRBF2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-73054] - Analysis of HT29 cell lysate (35ug).
Western Blot: NRBF2 Antibody (OTI1A2)Azide and BSA Free [NBP2-73054]

Western Blot: NRBF2 Antibody (OTI1A2)Azide and BSA Free [NBP2-73054]

Western Blot: NRBF2 Antibody (OTI1A2) - Azide and BSA Free [NBP2-73054] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBF2.

Applications for NRBF2 Antibody (OTI1A2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NRBF2

NRBF2 (nuclear receptor binding factor-2) was originally identified in a yeast two-hybrid screen for factors that interact with the peroxisome proliferator-activated receptor alpha (PPARalpha). In a second study, NRBF2 was also identified in a screen of a keratinocyte cDNA library and named COPR (comodulator of PPAR and RXR). In this study, NRBF2 was proposed to function as a modulator of transcriptional activity that functions to decrease rather than completely repress nuclear-receptor mediated gene expression. Alternative names for NRBF2 include COPR1, COPR2, and NRBF-2.

Alternate Names

Comodulator of PPAR and RXR, COPR, COPR1, COPR2, DKFZp564C1664, FLJ30395, NRBF-2, nuclear receptor binding factor 2, nuclear receptor binding factor-2, nuclear receptor-binding factor 2

Gene Symbol

NRBF2

Additional NRBF2 Products

Product Documents for NRBF2 Antibody (OTI1A2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NRBF2 Antibody (OTI1A2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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