Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NRF1/2609

Concentration

0.2 mg/ml

View all Nrf1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Nrf1 protein (Uniprot: Q16656)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08773)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Nrf1 Antibody (NRF1/2609)

Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Western Blot: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Western Blot Analysis of human HEK293, HeLa, A431, MOLT4 cell lysates using Nrf1 Mouse Monoclonal Antibody (NRF1/2609).
Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).
Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451]

Immunohistochemistry-Paraffin: Nrf1 Antibody (NRF1/2609) [NBP3-07451] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Nrf1 Mouse Monoclonal Antibody (Nrf1/2609).

Applications for Nrf1 Antibody (NRF1/2609)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Nrf1

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Gene Symbol

NRF1

UniProt

Q16656 (Human)

Additional Nrf1 Products

Product Documents for Nrf1 Antibody (NRF1/2609)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nrf1 Antibody (NRF1/2609)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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