Nrf1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77822
Key Product Details
Species Reactivity
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This protein A purified Nrf1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse Nrf1 protein corresponding to aa 1- 534 of the native protein. (Uniprot: Q9WU00)
Reactivity Notes
BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, rat, chicken, frog and zebrafish based on very high sequence homology with the immunogen sequence. Cross reactivity with Nrf1 homologues from other sources has not been determined.
Specificity
This protein A purified antibody is directed against mouse Nrf1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This protein A purified antibody is directed against mouse Nrf1. The product was purified from monospecific antiserum by protein A affinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Nrf1 Antibody
Western Blot: Nrf1 Antibody [NBP1-77822]
Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.Immunohistochemistry: Nrf1 Antibody [NBP1-77822]
Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.Western Blot: Nrf1 Antibody [NBP1-77822]
Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700Applications for Nrf1 Antibody
Application
Recommended Usage
ELISA
1:3000-1:10000
Immunohistochemistry
2 mg/ml-5 ug/ml
Immunohistochemistry-Paraffin
2 mg/ml-5 ug/ml
Western Blot
1:500-1:2000
Application Notes
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Nrf1
Long Name
NF-E2-related Factor 1
Alternate Names
HBZ17, LCR-F1, NFE2L1, TCF11
Gene Symbol
NRF1
UniProt
Additional Nrf1 Products
Product Documents for Nrf1 Antibody
Product Specific Notices for Nrf1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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