Nrf1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89125

Novus Biologicals, part of Bio-Techne
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NBP1-89125
NBP1-89125-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nrf1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTD

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31451517).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nrf1 Antibody

Western Blot: Nrf1 Antibody [NBP1-89125]

Western Blot: Nrf1 Antibody [NBP1-89125]

Western Blot: Nrf1 Antibody [NBP1-89125] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Nrf1 Antibody [NBP1-89125]

Western Blot: Nrf1 Antibody [NBP1-89125]

Western Blot: Nrf1 Antibody [NBP1-89125] - The SIAH2-NRF1 axis facilitates hypoxia-induced metabolic reprogramming. Wild-type or K230R stably expressed MDA-MB-231 cells were cultured under normoxia or hypoxia for 36 h. Cells were harvested and analyzed by western blotting with indicated antibodies. Right: densitometric quantification of the indicated proteins. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-08618-y), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody [NBP1-89125]

Immunocytochemistry/ Immunofluorescence: Nrf1 Antibody [NBP1-89125]

Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP1-89125] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
View 3 more images

Applications for Nrf1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nrf1

NRF1 encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Entrez Gene IDs

4899 (Human)

Gene Symbol

NRF1

Additional Nrf1 Products

Product Documents for Nrf1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nrf1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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