Nrf2 Antibody (2G7)
Novus Biologicals, part of Bio-Techne | Catalog # H00004780-M02
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Block/Neutralize, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2G7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NFE2L2 (AAH11558, 71 a.a. ~ 170 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCMQLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVA
Reactivity Notes
Mouse reactivity reported from customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
nuclear factor (erythroid-derived 2)-like 2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Nrf2 Antibody (2G7)
![Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02]](https://resources.bio-techne.com/images/products/Nrf2-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00004780-M02-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02]")
Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02]
Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell . Antibody concentration 10 ug/ml.![ELISA: Nrf2 Antibody (2G7) [H00004780-M02]](https://resources.bio-techne.com/images/products/Nrf2-Antibody-2G7-ELISA-H00004780-M02-img0006.jpg "ELISA: Nrf2 Antibody (2G7) [H00004780-M02]")
ELISA: Nrf2 Antibody (2G7) [H00004780-M02]
ELISA: Nrf2 Antibody (2G7) [H00004780-M02] - Detection limit for recombinant GST tagged NFE2L2 is approximately 3ng/ml as a capture antibody.
Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] -
Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. Cardiac expression of endogenous antioxidant-related genes was significantly reduced after TAC. Representative blot of NRF2 and quantitative results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080/) licensed under a CC-BY license.Applications for Nrf2 Antibody (2G7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 review rated 5 using H00004780-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nrf2
Long Name
NF-E2-related Factor 2
Alternate Names
NFE2L2
Entrez Gene IDs
4780 (Human)
Gene Symbol
NFE2L2
OMIM
600492 (Human)
UniProt
Additional Nrf2 Products
Product Documents for Nrf2 Antibody (2G7)
Product Specific Notices for Nrf2 Antibody (2G7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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