Nrf2 Antibody (JE30-14)

Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661]

Western Blot: Nrf2 Antibody (JE30-14) [NBP3-32661] - Western blot analysis of Nrf2 on different lysates with Rabbit anti-Nrf2 antibody (NBP3-32661) at 1/1,000 dilution.

Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 2uM MG-132 for 18 hours whole cell lysate
Lane 3: HCT 116 whole cell lysate
Lane 4: HCT 116 treated with 25uM MG-132 for 4 hours whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 68 kDa
Observed band size: 110 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32661) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.