Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NRIP Antibodies »

Product Specifications

Immunogen

Maps to a region between residue 300 and 350 of human nuclear receptor interaction protein (IQ motif and WD repeats 1) using the numbering given in entry NP_001017977.1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NRIP Antibody

Immunoprecipitation: NRIP Antibody [NBP1-30075]

Immunoprecipitation: NRIP Antibody [NBP1-30075]

Immunoprecipitation: NRIP Antibody [NBP1-30075] - Whole cell lysate from HeLa cells. NBP1-30075 used for IP at 10 mcg/mg lysate. NRIP was also immunoprecipitated by NBP1-30076. For blotting immunoprecipitated NRIP, NBP1-30076 was used at 1 mcg/ml.
NRIP Antibody

Immunohistochemistry-Paraffin: NRIP Antibody [NBP1-30075] -

Immunohistochemistry-Paraffin: NRIP Antibody [NBP1-30075] - FFPE section of human breast carcinoma.Antibody: Affinity purified rabbit anti- NRIP used at a dilution of 1:1,000 (1ug/ml). Detection:DAB

Applications for NRIP Antibody

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

10-15 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended, use rabbit anti-NRIP antibody NBP1-30076.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NRIP

WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies. Their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860a.a nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up-regulates their transcriptional activity, thereby functioning as a nuclear receptor co-activator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer, thus indicating a potential therapeutic intervention. Northern Blot analysis detects a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.

Alternate Names

1200006M05Rik, Androgen receptor complex-associated protein, ARCAP, DDB1 and CUL4 associated factor 6, DDB1- and CUL4-associated factor 6, FLJ23798, IQ motif and WD repeat-containing protein 1, IQ motif and WD repeats 1, IQWD1, MSTP055, NRIP, Nuclear receptor interaction protein, PC326

Entrez Gene IDs

55827 (Human)

Gene Symbol

DCAF6

UniProt

Q58WW2 (Human)

Additional NRIP Products

Product Documents for NRIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NRIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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