NSD3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33013
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NSD3 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NSD3. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NSD3 Antibody
Immunocytochemistry/ Immunofluorescence: NSD3 Antibody [NBP1-33013] -
Immunocytochemistry/ Immunofluorescence: NSD3 Antibody [NBP1-33013] - NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 um.
Western Blot: NSD3 Antibody [NBP1-33013] -
Western Blot: NSD3 Antibody [NBP1-33013] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 antibody [C1C2], Internal (NBP1-33013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.![Western Blot: NSD3 Antibody [NBP1-33013]](https://resources.bio-techne.com/images/products/NSD3-Antibody-Western-Blot-NBP1-33013-img0004.jpg "Western Blot: NSD3 Antibody [NBP1-33013]")
Western Blot: NSD3 Antibody [NBP1-33013]
Western Blot: NSD3 Antibody [NBP1-33013] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.Applications for NSD3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NSD3
Alternate Names
EC 2.1.1.43, FLJ20353, histone-lysine N-methyltransferase NSD3, MGC126766, MGC142029, NSD3DKFZp667H044, Nuclear SET domain-containing protein 3, pp14328, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, Wolf-Hirschhorn syndrome candidate 1-like 1, Wolf-Hirschhorn syndrome candidate 1-like protein 1
Entrez Gene IDs
54904 (Human)
Gene Symbol
NSD3
UniProt
Additional NSD3 Products
Product Documents for NSD3 Antibody
Product Specific Notices for NSD3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.