NSF Antibody (9G7-3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19333

Novus Biologicals, part of Bio-Techne
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NBP1-19333
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Primate, Rabbit, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 9G7-3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NSF Antibodies »

Product Specifications

Immunogen

Recombinant bovine NSF protein

Reactivity Notes

Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Pig, Rabbit, Sheep. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

NSF (9G7-3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for NSF Antibody (9G7-3)

Western Blot: NSF Antibody (9G7-3) [NBP1-19333]

Western Blot: NSF Antibody (9G7-3) [NBP1-19333]

Western Blot: NSF Antibody (9G7-3) [NBP1-19333] - Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.
Immunohistochemistry: NSF Antibody (9G7-3) [NBP1-19333]

Immunohistochemistry: NSF Antibody (9G7-3) [NBP1-19333]

Immunohistochemistry: NSF Antibody (9G7-3) [NBP1-19333] - Immunohistochemistry analysis of human testis tissue stained with NSF, mAb (9G7-3) at 20ug/ml.

Applications for NSF Antibody (9G7-3)

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

In PBS, pH 7.2, containing 50% glycerol.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NSF

N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.

Alternate Names

EC 3.6.4.6, NEM-sensitive fusion protein, N-ethylmaleimide-sensitive factor, N-ethylmaleimide-sensitive factor-like protein, N-ethylmaleimide-sensitive fusion protein, SKD2, vesicle-fusing ATPase, Vesicular-fusion protein NSF

Entrez Gene IDs

4905 (Human); 18195 (Mouse); 60355 (Rat)

Gene Symbol

NSF

Additional NSF Products

Product Documents for NSF Antibody (9G7-3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NSF Antibody (9G7-3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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