nSMase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87810

Novus Biologicals, part of Bio-Techne
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NBP1-87810
NBP1-87810-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Fish

Cited:

Fish

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all nSMase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHRGTPLSDHEALMATLFVRHSPPQQNPSST

Reactivity Notes

Fish reactivity reported in scientific literature (PMID: 31401171).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for nSMase Antibody

Western Blot: nSMase Antibody [NBP1-87810]

Western Blot: nSMase Antibody [NBP1-87810]

Western Blot: nSMase Antibody [NBP1-87810] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: nSMase Antibody [NBP1-87810]

Immunocytochemistry/ Immunofluorescence: nSMase Antibody [NBP1-87810]

Immunocytochemistry/Immunofluorescence: nSMase Antibody [NBP1-87810] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles. Antibody staining shown in green.
Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810]

Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810]

Immunohistochemistry-Paraffin: nSMase Antibody [NBP1-87810] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.
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Applications for nSMase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: nSMase

Sphingomyelin is also present in all eukaryotic cell membranes, especially the plasma membrane, and is particularly concentrated in the nervous system because sphingomyelin is a major component of myelin, the fatty insulation wrapped around nerve cells by Schwann cells or oligodendrocytes. Multiple Sclerosis is a disease characterised by deterioration of the myelin sheath, leading to impairment of nervous conduction. nSMase (Neutral sphingomyelinase; Sphingomyelin phosphodiesterase 2) converts sphingomyelin to ceramide. Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain. Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity.

Alternate Names

EC 3.1.4.12, ISC1, Lyso-PAF-PLC, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase, NSMASE, N-SMase, NSMASE1, sphingomyelin phosphodiesterase 2, sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)

Gene Symbol

SMPD2

Additional nSMase Products

Product Documents for nSMase Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for nSMase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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