NT-3 Antibody (OTI5A2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47892

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5A2

Concentration

1.5 mg/ml

View all NT-3 Antibodies »

Product Summary for NT-3 Antibody (OTI5A2)

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 139-257 of human NT3

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens neurotrophin 3 (NTF3), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NT-3 Antibody (OTI5A2)

Western Blot: NT-3 Antibody (OTI5A2) [NBP1-47892]

Western Blot: NT-3 Antibody (OTI5A2) [NBP1-47892]

Western Blot: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Neurotrophin 3 antibody (5A2) at 1:5000 + Mature Recombinant Human Neurotrophin 3 (14KDa).
Immunocytochemistry/Immunofluorescence: Neurotrophin 3 Antibody (5A2) [NBP1-47892] Staining of HeLa cells transiently transfected by pCMV6-ENTRY NTF3.
Immunohistochemistry-Paraffin: NT-3 Antibody (OTI5A2) [NBP1-47892]

Immunohistochemistry-Paraffin: NT-3 Antibody (OTI5A2) [NBP1-47892]

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded lung using anti-NTF3 mouse monoclonal antibody.
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Applications for NT-3 Antibody (OTI5A2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NT-3

NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse

Long Name

Neurotrophin 3

Alternate Names

HDNF, NGF2, NT3, NTF3

Entrez Gene IDs

4908 (Human)

Gene Symbol

NTF3

Additional NT-3 Products

Product Documents for NT-3 Antibody (OTI5A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NT-3 Antibody (OTI5A2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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