NT-4 Antibody (1G12)
Novus Biologicals, part of Bio-Techne | Catalog # H00004909-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NTF5 (AAH12421, 1 a.a. ~ 210 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA
Specificity
NTF5 - neurotrophin 5 (neurotrophin 4/5) (1G12)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NT-4 Antibody (1G12)
![Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02]](https://resources.bio-techne.com/images/products/NT-4-Antibody-1G12-Proximity-Ligation-Assay-H00004909-M02-img0001.jpg "Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02]")
Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02]
Proximity Ligation Assay: NT-4 Antibody (1G12) [H00004909-M02] - Analysis of protein-protein interactions between BDNF and NTF4. HeLa cells were stained with anti-BDNF rabbit purified polyclonal 1:1200 and anti-NTF4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: NT-4 Antibody (1G12) [H00004909-M02] - Detection limit for recombinant GST tagged NTF4 is 1 ng/ml as a capture antibody.
Applications for NT-4 Antibody (1G12)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NT-4
Long Name
Neurotrophin 4
Alternate Names
NT-5, NT4, NTF4, NTF5
Entrez Gene IDs
4909 (Human)
Gene Symbol
NTF4
UniProt
Additional NT-4 Products
Product Documents for NT-4 Antibody (1G12)
Product Specific Notices for NT-4 Antibody (1G12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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