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NUAK2 Antibody (2F9)

Novus Biologicals, part of Bio-Techne | Catalog # H00081788-M04

Novus Biologicals, part of Bio-Techne
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H00081788-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NUAK2 (NP_112214.1, 521 a.a. ~ 628 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FGSLDELAPPRPLARASRPSGAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPLGDSCFSLTDCQEVTATYRQALRVCSKLT

Specificity

Reacts with NUAK family, SNF1-like kinase, 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for NUAK2 Antibody (2F9)

Western Blot: NUAK2 Antibody (2F9) [H00081788-M04]

Western Blot: NUAK2 Antibody (2F9) [H00081788-M04]

Western Blot: NUAK2 Antibody (2F9) [H00081788-M04] - Analysis of NUAK2 expression in transfected 293T cell line by NUAK2 monoclonal antibody (M04), clone 2F9. Lane 1: NUAK2 transfected lysate (Predicted MW: 69.6 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NUAK2 Antibody (2F9) [H00081788-M04]

Immunocytochemistry/ Immunofluorescence: NUAK2 Antibody (2F9) [H00081788-M04]

Immunocytochemistry/Immunofluorescence: NUAK2 Antibody (2F9) [H00081788-M04] - Analysis of monoclonal antibody to NUAK2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: NUAK2 Antibody (2F9) [H00081788-M04] - Detection limit for recombinant GST tagged NUAK2 is 1 ng/ml as a capture antibody.

Applications for NUAK2 Antibody (2F9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NUAK2

Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment byincreasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF-alpha, via NF-kappa-B. Protects cellsfrom CD95-mediated apoptosis and is required for the increased motility and invasiveness of CD95-activated tumor cells

Long Name

NUAK family SNF1-like kinase 2

Alternate Names

OMPHK2, SNARK

Entrez Gene IDs

81788 (Human)

Gene Symbol

NUAK2

Additional NUAK2 Products

Product Documents for NUAK2 Antibody (2F9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NUAK2 Antibody (2F9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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