NUAK2 Antibody

Western Blot: NUAK2 Antibody [NBP1-81880]

Western Blot: NUAK2 Antibody [NBP1-81880] - Analysis in control (vector only transfected HEK293T lysate) and NUAK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880]

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880]

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human cervix shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880]

Immunohistochemistry-Paraffin: NUAK2 Antibody [NBP1-81880] - Staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.

Knockdown Validated: NUAK2 Antibody [NBP1-81880]

Knockdown Validated: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, and target gene expression in MDA-MB231 cells. YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-05939-2), licensed under a CC-BY license.

Western Blot: NUAK2 Antibody [NBP1-81880] -

Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 activity is involved in serum-induced or LPA-induced YAP/TAZ dephosphorylation & activation. a, b FBS & LPA activate YAP/TAZ & induce NUAK2 expression. YAP/TAZ phosphorylation status was monitored using Phos-Tag gels (a), while NUAK2 & ANKRD1 mRNA expression was determined by qPCR at the indicated times. Expression data are plotted as the mean ± SD (n = 3). c–h WZ4003 (10 μM) blocks serum/LPA-induced NUAK2 & ANKRD1 mRNA expression. FBS-induced or LPA-induced YAP/TAZ dephosphorylation (e & g), or NUAK2 & ANKRD1 mRNA expression (c, f, h) was monitored at the indicated times (d). Expression data are plotted as the mean ± range for a representative experiment (c, f, h) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NUAK2 Antibody [NBP1-81880] -

Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NUAK2 Antibody [NBP1-81880] -

Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NUAK2 Antibody [NBP1-81880] -

Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 is associated with high-grade human bladder cancer. a, c Expression of NUAK2 & the YAP Signature Genes (YAP SG) is enhanced both in high-grade non-muscle-invasive (HG-NMIBC; n = 13, **p = 0.0071) & muscle-invasive (pT2 MIBC; n = 9, **p = 0.0033) bladder cancers39 as compared to LG-NMIBC (n = 27) using an unpaired t test. b Elevated expression of NUAK2 is correlated with disease recurrence in a cohort41 of MIBC patient samples with n = 57 disease free & n = 56, recurred samples (*p = 0.0172). Box & whisker plots show the median (line in the box), first & third quartiles (lower & upper ends of the box), & the minimum & maximum values (whiskers in the plot). Dots represent a single patient sample. d A heat map depicting expression of NUAK2 & YAP SG in NMIBC LG (open circle), HG (closed circle), & MIBC pT2 (slashed circle) bladder cancer samples39. e Characterization of high-grade-derived & low-grade-derived bladder cancer cell lines. Scale bar, 25 μm. f NUAK2 mRNA expression is elevated in HG-derived (TCCSUP & T24) BC cell lines as compared to LG lines. Data are plotted as the mean ± SD (n = 3). g–i Blocking NUAK2 activity with 10 μM WZ4003 (g) or NUAK2 expression in stable clones (#5 or #7) using inducible NUAK2 shRNAs (h, i) inhibits the growth of HG BC cell lines as measured by SRB (g) or DAPI (h) staining. Data are plotted as the mean ± SD of a representative experiment, n = 3 (for TCC) or mean ± range of two experiments (for T24) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NUAK2 Antibody [NBP1-81880] -

Western Blot: NUAK2 Antibody [NBP1-81880] - NUAK2 regulates YAP/TAZ activity through LATS. a Loss of NUAK2 enhances YAP/TAZ phosphorylation in MDA-MB231 cells. Quantitation of relative phosphorylation levels from blots is shown. b NUAK2 interacts with LATS1 & LATS2, but not MST1, MST2, or TAZ in the LUMIER protein interaction screen. c Lysates from HEK293T cells, transfected with NUAK2-Flag, were subjected to immunoprecipitation (IP) using anti-LATS1 antibody for endogenous LATS1 & co-immunoprecipitated NUAK2-Flag was detected by immunoblotting. Protein expression levels were confirmed (Totals). WT wild type, KR K81R, kinase dead. d–f NUAK2 requires LATS to regulate YAP/TAZ localization, phosphorylation, & target gene expression in MDA-MB231 cells. d YAP/TAZ localization was quantitated & plotted as the mean ± SD (n = 3) with representative images shown on the right. Scale bars, 25 μm. N: nuclear, C: cytoplasmic. e Target gene expression was determined by qPCR. Data are plotted as the mean ± SD (n = 3). f YAP/TAZ phosphorylation was monitored using Phos-Tag gels. Relative phosphorylation levels from blots is quantitated (right) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30158528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.