NUB1/NYREN18 Antibody (OTI4H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03897

Novus Biologicals, part of Bio-Techne
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NBP2-03897
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4H2

Concentration

1 mg/ml

View all NUB1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NUB1/NYREN18 Antibody (OTI4H2)

Western Blot: NYREN18 Antibody (4H2) [NBP2-03897] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NYREN18 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: NUB1/NYREN18 Antibody (OTI4H2) [NBP2-03897]

Immunocytochemistry/ Immunofluorescence: NUB1/NYREN18 Antibody (OTI4H2) [NBP2-03897]

Immunocytochemistry/Immunofluorescence: NYREN18 Antibody (4H2) [NBP2-03897] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NYREN18.
Immunohistochemistry-Paraffin: NUB1/NYREN18 Antibody (OTI4H2) [NBP2-03897]

Immunohistochemistry-Paraffin: NUB1/NYREN18 Antibody (OTI4H2) [NBP2-03897]

Immunohistochemistry-Paraffin: NYREN18 Antibody (4H2) [NBP2-03897] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NYREN18 mouse monoclonal antibody.
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Applications for NUB1/NYREN18 Antibody (OTI4H2)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUB1

NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members.[supplied by OMIM].

Long Name

NEDD8 Ultimate Buster 1

Alternate Names

BS4, NUB1L, NY-REN-18

Entrez Gene IDs

51667 (Human)

Gene Symbol

NUB1

Additional NUB1 Products

Product Documents for NUB1/NYREN18 Antibody (OTI4H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NUB1/NYREN18 Antibody (OTI4H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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