NucA nuclease Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB100633
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # 1028905
Product Specifications
Immunogen
E. coli-derived s. marcescens NucA nuclease
Met1-Asn266
Accession # P13717
Met1-Asn266
Accession # P13717
Specificity
Detects NucA nuclease in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NucA nuclease Antibody
Detection of NucA nuclease by Western Blot.
Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of NucA nuclease by Simple WesternTM.
Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for NucA nuclease Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Recombinant NucA nuclease Protein
Sample: Recombinant NucA nuclease Protein
Western Blot
2 µg/mL
Sample: Recombinant NucA nuclease Protein
Sample: Recombinant NucA nuclease Protein
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NucA nuclease
Long Name
NucA nuclease
Alternate Names
DcD
Entrez Gene IDs
23387471 (Bacterial)
Gene Symbol
SMDB11_RS05280
UniProt
Additional NucA nuclease Products
Product Documents for NucA nuclease Antibody
Product Specific Notices for NucA nuclease Antibody
For research use only
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