NucA nuclease Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10063
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Multi-Species
Applications
ELISA, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2404B
Product Specifications
Immunogen
E. coli-derived S. marcescens recombinant NucA nuclease
Met1-Asn266
Accession # P13717
Met1-Asn266
Accession # P13717
Specificity
Detects NucA nuclease in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NucA nuclease Antibody
NucA nuclease ELISA Standard Curve.
Recombinant NucA nuclease was serially diluted 2-fold and captured by Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-NucA nuclease Monoclonal Antibody (MAB100632) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Detection of NucA nuclease by Western Blot.
Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of NucA nuclease by Simple WesternTM.
Simple Western lane view shows lysates of recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB10063). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for NucA nuclease Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100632).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Simple Western
10 µg/mL
Sample: Recombinant NucA nuclease Protein
Sample: Recombinant NucA nuclease Protein
Western Blot
2 µg/mL
Sample: Recombinant NucA nuclease Protein
Sample: Recombinant NucA nuclease Protein
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NucA nuclease
Long Name
NucA nuclease
Alternate Names
DcD
Entrez Gene IDs
23387471 (Bacterial)
Gene Symbol
SMDB11_RS05280
UniProt
Additional NucA nuclease Products
Product Documents for NucA nuclease Antibody
Product Specific Notices for NucA nuclease Antibody
For research use only
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