Nucleobindin 1 Antibody (OTI1A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01446

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A5

Concentration

0.93 mg/ml

View all Nucleobindin 1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleobindin 1 Antibody (OTI1A5)

Western Blot: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Western Blot: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Western Blot: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1.
Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (OTI1A5) [NBP2-01446]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody (1A5) [NBP2-01446] - Staining of paraffin-embedded Human endometrium tissue using anti-Nucleobindin 1 mouse monoclonal antibody.
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Applications for Nucleobindin 1 Antibody (OTI1A5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.93 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nucleobindin 1

This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq].

Alternate Names

DKFZp686A15286, FLJ40471, NUCCALNUC, nucleobindin 1, nucleobindin-1

Entrez Gene IDs

4924 (Human)

Gene Symbol

NUCB1

Additional Nucleobindin 1 Products

Product Documents for Nucleobindin 1 Antibody (OTI1A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleobindin 1 Antibody (OTI1A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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