Nucleobindin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88217

Novus Biologicals, part of Bio-Techne
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NBP1-88217
NBP1-88217-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nucleobindin 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nucleobindin 1 Antibody

Western Blot: Nucleobindin 1 Antibody [NBP1-88217]

Western Blot: Nucleobindin 1 Antibody [NBP1-88217]

Western Blot: Nucleobindin 1 Antibody [NBP1-88217] - Analysis in human cell line SiHa.
Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP1-88217]

Immunocytochemistry/ Immunofluorescence: Nucleobindin 1 Antibody [NBP1-88217]

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody [NBP1-88217] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217]

Immunohistochemistry-Paraffin: Nucleobindin 1 Antibody [NBP1-88217] - Staining in human small intestine and pancreas tissues using NBP1-88217 antibody. Corresponding NUCB1 RNA-seq data are presented for the same tissues.
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Applications for Nucleobindin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleobindin 1

Nucleobindin 1 is a major calcium binding protein of the Golgi. It may have a role in calcium homeostasis.

Alternate Names

DKFZp686A15286, FLJ40471, NUCCALNUC, nucleobindin 1, nucleobindin-1

Gene Symbol

NUCB1

Additional Nucleobindin 1 Products

Product Documents for Nucleobindin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleobindin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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