Nucleolin Antibody (364-5) - Azide and BSA Free

Validated:

Unconjugated

Monoclonal Mouse IgG₁ kappa Clone # 364-5

Azide and BSA Free

1.0 mg/ml

lysate of SU-DHL-1 Nuclei (Uniprot: P19338)

Does not react with Mouse, Rat or Bovine.

Nucleoli

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Marker of Human Cells

Monoclonal

Mouse

IgG₁ kappa

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44612).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Protein A or G purified

10 mM PBS

Azide and BSA Free

No Preservative

1.0 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20 to -80C. Avoid freeze-thaw cycles.