Nucleolin Antibody (NCL/8068R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24068
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # NCL/8068R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Nucleolin protein
Localization
Nucleoli
Specificity
This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Marker
Marker of Human Cells
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for Nucleolin Antibody (NCL/8068R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Nucleolin Antibody (NCL/8068R) - Azide and BSA Free [NBP3-24068] -
Formalin-fixed, paraffin-embedded human colon cancer stained with Nucleolin antibody (NCL/8068R) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Nucleolin Antibody (NCL/8068R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Western Blot
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Nucleolin
Alternate Names
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbol
NCL
Additional Nucleolin Products
Product Documents for Nucleolin Antibody (NCL/8068R) - Azide and BSA Free
Product Specific Notices for Nucleolin Antibody (NCL/8068R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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