Nucleolin Antibody (SPM614) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48318
![Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) - IHC-Prediluted [NBP2-48318]](https://resources.bio-techne.com/images/products/Nucleolin-Antibody-SPM614-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48318-img0001.jpg "Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) - IHC-Prediluted [NBP2-48318]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM614
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Nucleolin Antibody (SPM614) - IHC-Prediluted
Immunogen
Recombinant full-length human Nucleolin protein (Uniprot: P19338)
Reactivity Notes
Does not react with Mouse, Rat or Bovine.
Localization
Nucleoli
Specificity
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Marker
Marker of Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Nucleolin Antibody (SPM614) - IHC-Prediluted
Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) - IHC-Prediluted [NBP2-48318]
Immunohistochemistry-Paraffin: Nucleolin Antibody (SPM614) - IHC-Prediluted [NBP2-48318] - Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (SPM614).Applications for Nucleolin Antibody (SPM614) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 2q37.1 Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Nucleolin
Alternate Names
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Gene Symbol
NCL
Additional Nucleolin Products
Product Documents for Nucleolin Antibody (SPM614) - IHC-Prediluted
Product Specific Notices for Nucleolin Antibody (SPM614) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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