Nucleolin [p Thr76] Antibody (SR1019)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21607

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21607-100ul
NBP3-21607-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1019

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nucleolin Antibodies »

Product Summary for Nucleolin [p Thr76] Antibody (SR1019)

Immunogen

A synthesized peptide derived from human phospho-Nucleolin [Thr76] (Uniprot #: P19338)

Modification

p Thr76

Specificity

Detects endogenous levels of total phospho-Nucleolin [Thr76]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleolin [p Thr76] Antibody (SR1019)

Western Blot: Nucleolin [p Thr 76] Antibody (SR1019) [NBP3-21607] -

Western Blot: Nucleolin [p Thr 76] Antibody (SR1019) [NBP3-21607] - Western blot analysis using NBP3-21607 in (1) 293T cell lysate; (2) 293T cell treated with Lambda Phosphatase lysate.

Applications for Nucleolin [p Thr76] Antibody (SR1019)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nucleolin

Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein. It is the major protein in actively dividing cells, whereas the degraded forms are relatively abundant in non-dividing cells. Stability of the nucleolin protein is cell proliferation-dependent. It plays a role in rDNA transcription, organization, and rRNA processing. It may alter DNA organization in response to cell cycle controls. Nucleolin antibodies are commonly used as a mammalian nucleolus marker.

Alternate Names

C23, FLJ45706, FLJ59041, nucleolin, Protein C23

Gene Symbol

NCL

Additional Nucleolin Products

Product Documents for Nucleolin [p Thr76] Antibody (SR1019)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleolin [p Thr76] Antibody (SR1019)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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