Nucleoporin NUP85 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1355
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ARAIIREGSLEGS corresponding to C-Terminus according to NP_079120.1.
Localization
Nuclear
Specificity
Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Nucleoporin NUP85 Antibody
![Western Blot: Nucleoporin NUP85 Antibody [NB100-1355]](https://resources.bio-techne.com/images/products/NB100-1355_Western-Blot-Nucleoporin-NUP85-Antibody-NB100-1355-1ug-ml-staining-of-Hela-lysate-35ug-protein-in-RIPA-buffer-Detected-by-chemiluminescence--29320239254032.jpg "Western Blot: Nucleoporin NUP85 Antibody [NB100-1355]")
Western Blot: Nucleoporin NUP85 Antibody [NB100-1355]
Western Blot: Nucleoporin NUP85 Antibody [NB100-1355] - (1ug/ml) staining of Hela lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for Nucleoporin NUP85 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa band according to NP_079120).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Nucleoporin NUP85
Long Name
Nuclear Pore Complex Protein Nup85
Alternate Names
FROUNT, Nup75, NUP85, PCNT1, Pericentrin-1
Gene Symbol
NUP85
UniProt
Additional Nucleoporin NUP85 Products
Product Documents for Nucleoporin NUP85 Antibody
Product Specific Notices for Nucleoporin NUP85 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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