Nucleotide binding protein like Antibody (OTI5D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01360

Novus Biologicals, part of Bio-Techne
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NBP2-01360
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-73094AF350
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Alexa Fluor 405
NBP2-73094AF405
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Alexa Fluor 488
NBP2-73094AF488
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Alexa Fluor 532
NBP2-73094AF532
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Alexa Fluor 594
NBP2-73094AF594
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Alexa Fluor 647
NBP2-73094AF647
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Alexa Fluor 700
NBP2-73094AF700
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Alexa Fluor 750
NBP2-73094AF750
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Allophycocyanin
NBP2-73094APC
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Allophycocyanin/Cy7
NBP2-73094APCCY7
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Biotin
NBP2-73094B
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CoraFluor 1
NBP2-73094CL1
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DyLight 350
NBP2-73094UV
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DyLight 405
NBP2-73094V
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DyLight 488
NBP2-73094G
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DyLight 550
NBP2-73094R
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DyLight 594
NBP2-73094DL594
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DyLight 650
NBP2-73094C
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DyLight 680
NBP2-73094FR
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DyLight 755
NBP2-73094IR
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FITC
NBP2-73094F
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HRP
NBP2-73094H
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Janelia Fluor 525
NBP2-73094JF525
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Janelia Fluor 549
NBP2-73094JF549
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Janelia Fluor 585
NBP2-73094JF585
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Janelia Fluor 635
NBP2-73094JF635
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Janelia Fluor 646
NBP2-73094JF646
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Janelia Fluor 669
NBP2-73094JF669
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mFluor Violet 450 SE
NBP2-73094MFV450
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mFluor Violet 500 SE
NBP2-73094MFV500
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mFluor Violet 610 SE
NBP2-73094MFV610
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PE
NBP2-73094PE
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PE/Atto594
NBP2-73094PEATT594
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PE/Cy5.5
NBP2-73094PECY55
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PE/Cy7
NBP2-73094PECY7
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PerCP
NBP2-73094PCP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI5D5

Concentration

1.1 mg/ml

View all Nucleotide binding protein like Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-250 of human NUBPL(NP_079428) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

33.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nucleotide binding protein like Antibody (OTI5D5)

Western Blot: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Western Blot: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Western Blot: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nucleotide binding protein like (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nucleotide binding protein like.
Immunocytochemistry/ Immunofluorescence: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Immunocytochemistry/ Immunofluorescence: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Immunocytochemistry/Immunofluorescence: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleotide binding protein like.
Immunohistochemistry-Paraffin: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Immunohistochemistry-Paraffin: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360]

Immunohistochemistry-Paraffin: Nucleotide binding protein like Antibody (OTI5D5) [NBP2-01360] - Staining of paraffin-embedded Human tonsil using anti-Nucleotide binding protein like mouse monoclonal antibody.
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Applications for Nucleotide binding protein like Antibody (OTI5D5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nucleotide binding protein like

This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. The respiratory complex I consists of 45 subunits and 8 iron-sulfur (Fe/S) clusters. This protein is an Fe/S protein that plays a critical role in the assembly of respiratory complex I, likely by transferring Fe/S into the Fe/S-containing complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Alternate Names

C14orf127, FLJ12660, huInd1IND1 homolog, IND1chromosome 14 open reading frame 127, iron-sulfur protein NUBPL, iron-sulfur protein required for NADH dehydrogenase, nucleotide binding protein-like, Nucleotide-binding protein-like

Entrez Gene IDs

80224 (Human)

Gene Symbol

NUBPL

Additional Nucleotide binding protein like Products

Product Documents for Nucleotide binding protein like Antibody (OTI5D5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nucleotide binding protein like Antibody (OTI5D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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