NUDC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51971

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NUDC Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PNLGNGADLPNYR corresponding to internal region according to NP_006591.1.

Epitope

C-PNLGNGADLPNYR

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NUDC Antibody

Western Blot: NUDC Antibody [NBP1-51971]

Western Blot: NUDC Antibody [NBP1-51971]

Western Blot: NUDC Antibody [NBP1-51971] - (0.5ug/ml) staining of HeLa (A), HepG2 (B), K562 (C) and KNRK (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NUDC Antibody [NBP1-51971]

Immunocytochemistry/ Immunofluorescence: NUDC Antibody [NBP1-51971]

Immunocytochemistry/Immunofluorescence: NUDC Antibody [NBP1-51971] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NUDC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx 40kDa band observed in lysates of cell lines HeLa. HepG2 and K562, and approx. 38kDa in lysates of cell line KNRK (calculated MW of 38.2kDa according to Human NP_006591.1) and 38.4kDa according to Rat NP_058967.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NUDC

NudC was first identified as a regulator of nuclear movement in the asexual reproductive cycle of the filamentous fungus Aspergillus nidulans. Human NUDC is a nuclear movement protein that associates with dynein (see DYNC1H1; MIM 600112) (Aumais et al., 2003 [PubMed 12679384]).[supplied by OMIM]

Alternate Names

HNUDC, MNUDC, NPD011, nuclear distribution gene C (A.nidulans) homolog, nuclear distribution gene C homolog (A. nidulans), Nuclear distribution protein C homolog, nuclear migration protein nudC, NudC

Entrez Gene IDs

10726 (Human); 18221 (Mouse); 29648 (Rat)

Gene Symbol

NUDC

UniProt

NP_006591.1 (Human)

Additional NUDC Products

Product Documents for NUDC Antibody

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Certificate of Analysis

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Product Specific Notices for NUDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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