NUDCD2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06434
![Western Blot: NUDCD2 Antibody [NBP3-06434]](https://resources.bio-techne.com/images/products/NUDCD2-Antibody-Western-Blot-NBP3-06434-img0003.jpg "Western Blot: NUDCD2 Antibody [NBP3-06434]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human NUDCD2 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for NUDCD2 Antibody
Western Blot: NUDCD2 Antibody [NBP3-06434]
Western Blot: NUDCD2 Antibody [NBP3-06434] - NUDCD2 was immunoprecipitated using: Lane A:0.5 mg K562 Whole Cell Lysate. 4 uL anti-NUDCD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NUDCD2 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :18 kDa.![Immunocytochemistry/ Immunofluorescence: NUDCD2 Antibody [NBP3-06434]](https://resources.bio-techne.com/images/products/NUDCD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06434-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NUDCD2 Antibody [NBP3-06434]")
Immunocytochemistry/ Immunofluorescence: NUDCD2 Antibody [NBP3-06434]
Immunocytochemistry/Immunofluorescence: NUDCD2 Antibody [NBP3-06434] - Immunofluorescence staining of NUDCD2 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NUDCD2 polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.![Western Blot: NUDCD2 Antibody [NBP3-06434]](https://resources.bio-techne.com/images/products/NUDCD2-Antibody-Western-Blot-NBP3-06434-img0001.jpg "Western Blot: NUDCD2 Antibody [NBP3-06434]")
Western Blot: NUDCD2 Antibody [NBP3-06434]
Western Blot: NUDCD2 Antibody [NBP3-06434] - Anti-NUDCD2 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:18 kDa.Applications for NUDCD2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NUDCD2
Alternate Names
DKFZp686E10109, NudC domain containing 2, nudC domain-containing protein 2, NudC-like protein 2
Gene Symbol
NUDCD2
Additional NUDCD2 Products
Product Specific Notices for NUDCD2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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