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Nudel Antibody (OTI5E11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01086

Novus Biologicals, part of Bio-Techne
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NBP2-01086

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5E11

Concentration

0.6 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human NDEL1(NP_001020750) produced in HEk293 cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nudel Antibody (OTI5E11)

Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Nudel Antibody (OTI5E11) [NBP2-01086]

Immunocytochemistry/ Immunofluorescence: Nudel Antibody (OTI5E11) [NBP2-01086]

Immunocytochemistry/Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.
Immunohistochemistry-Paraffin: Nudel Antibody (OTI5E11) [NBP2-01086]

Immunohistochemistry-Paraffin: Nudel Antibody (OTI5E11) [NBP2-01086]

Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.

Applications for Nudel Antibody (OTI5E11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nudel

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Alternate Names

DKFZp451M0318, EOPA, MITAP1mitosin-associated protein MITAP1, Mitosin-associated protein 1, nuclear distribution protein nudE-like 1, nudE nuclear distribution gene E homolog (A. nidulans)-like 1, nudE nuclear distribution gene E homolog like 1, nudE nuclear distribution gene E homolog like 1 (A. nidulans), NUDELendooligopeptidase A, NUDE-like protein, Protein Nudel

Entrez Gene IDs

81565 (Human)

Gene Symbol

NDEL1

Additional Nudel Products

Product Documents for Nudel Antibody (OTI5E11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nudel Antibody (OTI5E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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