Numb Antibody (9X1Y0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16738

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16738-20ul
NBP3-16738-100ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9X1Y0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Numb Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NUMB (P49757). MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Numb Antibody (9X1Y0)

Western Blot: Numb Antibody (9X1Y0) [NBP3-16738]

Western Blot: Numb Antibody (9X1Y0) [NBP3-16738]

Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738]

Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738]

Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Numb antibody (NBP3-16738). Western blot was performed from the immunoprecipitate using Numb (NBP3-16738) at a dilition of 1:1000.

Applications for Numb Antibody (9X1Y0)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Numb

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Alternate Names

m-numb, mnb, S171

Gene Symbol

NUMB

Additional Numb Products

Product Documents for Numb Antibody (9X1Y0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Numb Antibody (9X1Y0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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