Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B5

Concentration

1 mg/ml

View all Numb Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Numb Antibody (OTI1B5)

Western Blot: Numb Antibody (OTI1B5) [NBP2-46284]

Western Blot: Numb Antibody (OTI1B5) [NBP2-46284]

Western Blot: Numb Antibody (1B5) [NBP2-46284] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB.
Immunohistochemistry: Numb Antibody (OTI1B5) [NBP2-46284]

Immunohistochemistry: Numb Antibody (OTI1B5) [NBP2-46284]

Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Numb Antibody (OTI1B5) [NBP2-46284]

Immunohistochemistry: Numb Antibody (OTI1B5) [NBP2-46284]

Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 5 more images

Applications for Numb Antibody (OTI1B5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Numb

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Alternate Names

m-numb, mnb, S171

Gene Symbol

NUMB

Additional Numb Products

Product Documents for Numb Antibody (OTI1B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Numb Antibody (OTI1B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.